Congratulations on your purchase of our amazing All-In-One grow bag!

Please read through this manual before proceeding. It contains important information that will enhance your experience with our product.

We take great care in packaging our products, ensuring their safe journey to you. However, on rare occasions, items may experience some damage during transit. Therefore, we kindly request you to thoroughly inspect the bag for any signs of damage that might have occurred during transportation. Please don't hesitate to reach out to us immediately if you spot any damage. We will promptly issue a replacement.

Before the injection

Our all-in-one bags undergo a critical sterilisation process, where they are sterilised for a duration of 2.5 hours at 15psi / 121°C. After sterilisation process is finished, we incubate our All-In-One bags for a minimum of 10 days to ensure that all our All-In-One bags are 100% sterile.

Please check your bag for any signs of contamination and store the bag at room temperature for at least 7 days before injection to confirm the sterility of the bag.

If you encounter contamination at a later stage of your cultivation process, it is most likely due to a contaminated spore syringe, mushroom culture, or inadequate sterile techniques. We cannot provide the replacement All-In-One bags or issue refunds after injection.

Spore syringes VS Liquid culture

Mushroom liquid culture (LC) offers several advantages over spore syringes, primarily due to its higher success rate. While it is possible to use mushroom spore syringes successfully, the success rate is much lower than 100% because it is practically impossible to produce absolutely sterile spore syringes due to the nature of how they are being made.

During the process of making spore syringes, spores are collected from the gills of mature mushrooms and suspended in a sterile liquid solution. However, despite the best efforts to maintain a sterile environment, it is nearly impossible to eliminate all potential contaminants. The collection process itself introduces the possibility of microbial or fungal spores being unintentionally mixed with the desired mushroom spores. The spores may also come into contact with airborne contaminants during the handling and preparation stages. These factors contribute to the inability to achieve absolute sterility in spore syringes, leading to a lower success rate.

Inoculation

Once your grow bag has been kept at room temperature for at least 7 days and sterility is confirmed, it can now be inoculated. Please read all steps thorough before you proceed.

1. Prepare your work area: Ensure that your work area is clean and free from contaminants. Consider working in a still air box (SAB) and wearing clean clothes for optimal cleanliness.

2. Sanitise your hands and tools: Wash your hands thoroughly with soap and wipe them down with blue Clinell skin wipe. Wipe down the injection port and surrounding area and syringe with a green Clinell wipe provided. 


3. Sterilise the needle: Hold the needle above a lighter flame until it turns red-hot. Allow it to cool for a few seconds. You can also cool the needle down by squirting some liquid from the syringe. 


4. Inoculate the All-In-One grow bag: Carefully inject 1-2ml of liquid culture (recommended) or spores through self-healing injection port into the rye grain.

Remember, maintaining a sterile environment is crucial to successful mushroom cultivation.

Colonisation

1. Keep the All-In-One bag in a clean environment at room temperature, ideally in the dark. After inoculating rye grain with mushroom spores or liquid culture, the mycelium typically starts to show visible signs of growth within 7 to 14 days. However, it's important to note that the exact timing can vary depending on various factors such as the mushroom species, temperature, humidity, and the quality of mushroom genetics used for inoculation. Patience is key during this stage, as mycelium growth may take slightly longer or show up earlier than the average timeframe.


2. Wait for the mushroom mycelium to colonise around 50% of the rye grain: Once half of the rye grain is colonised, it is time to break up rye grain and mix it with the substrate without opening the bag.


3. Wait until whole bag gets fully colonised: Your All-In-One grow bag now is ready for fruiting.

Fruiting

Once your All-In-One grow bag is fully colonised with mushroom mycelium it’s time to introduce the fruiting conditions which will initiate mushroom ‘pinning’. Different mushroom species have different requirements, but most of them require fresh air exchange, humidity and light. We recommend to check what are the best fruiting conditions for the mushroom variety you are growing.

Here are two fruiting methods:

1. Fruiting in a monotub (recommended): Monotub is a transparent plastic container with a lid, such as storage box. To fruit in a monotub, simply empty All-In-One grow bag contents to a monotub, lightly mist inside walls with clean water and loosely cover it with a lid leaving a gap for fresh air exchange. Keep the box in ambient light, away from direct sunlight. Ventilate the box once a day and mist the walls once water droplets have evaporated.


2. Fruiting directly from a bag: To introduce fruiting conditions cut the top of the bag just under the heat-seal line, lightly mist inside walls with clean water and fold the top of the bag. Use a paperclip or a peg to make sure the top of they bag stays folded. Next, puncture some holes above the substrate with a needle for fresh air exchange. Keep the bag in ambient light, away from direct sunlight. Ventilate the bag once a day and mist the inside walls once water droplets have evaporated.